RXR alpha/NR2B1 Antibody

Novus Biologicals | Catalog # NBP2-20130

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (97%), Mouse (99%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RXR alpha/NR2B1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RXR alpha/NR2B1 Antibody

Western Blot: RXR alpha/NR2B1 Antibody [NBP2-20130]

Western Blot: RXR alpha/NR2B1 Antibody [NBP2-20130]

Western Blot: Retinoid X Receptor alpha Antibody [NBP2-20130] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:5000.
RXR alpha/NR2B1 Antibody

Immunoprecipitation: RXR alpha/NR2B1 Antibody [NBP2-20130] -

Retinoid X Receptor alpha antibody immunoprecipitates RXRA protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of RXRA protein by 4 ug Retinoid X Receptor alpha antibody (NBP2-20130)
7.5 % SDS-PAGE
The immunoprecipitated RXRA protein was detected by Retinoid X Receptor alpha antibody (NBP2-20130) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
RXR alpha/NR2B1 Antibody

Western Blot: RXR alpha/NR2B1 Antibody [NBP2-20130] -

Rat tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Retinoid X Receptor alpha antibody (NBP2-20130) diluted at 1:500.

Applications for RXR alpha/NR2B1 Antibody

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RXR alpha/NR2B1

Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. [provided by RefSeq]

Long Name

Retinoid X Receptor, alpha

Alternate Names

NR2B1, RXRA

Gene Symbol

RXRA

UniProt

Additional RXR alpha/NR2B1 Products

Product Documents for RXR alpha/NR2B1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RXR alpha/NR2B1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for RXR alpha/NR2B1 Antibody

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