S-Tag Epitope Tag Antibody (247) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43726

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 247

Format

Azide and BSA Free
View all S-Tag Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to S-Tag Epitope Tag

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for S-Tag Epitope Tag Antibody (247) - Azide and BSA Free

Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726]

Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726]

Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726] - Analysis of A. 30 ug 293T whole cell lysate/eztract B. 30 ug whole cell lysate/extract of S-tagged protein expressing 293T cells 12 % SDS-PAGE S Tag antibody dilution: 1:5000.
Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726]

Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726]

Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726] - Analysis of 400 ug cell lysate/extract of S-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of S-fused protein by 2 ug of S tag antibody 12% SDS-PAGE The immunoprecipitated S-fused protein was detected by S tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.

Applications for S-Tag Epitope Tag Antibody (247) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1:500-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: S-Tag Epitope Tag

The S-Tag epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized S-Tag epitope represents the amino acid sequence KETAAAKFERQHMDS derived from pancreatic ribonuclease A.

Alternate Names

KETAAAKFERQHMDS, S 7 Epitope tag

Additional S-Tag Epitope Tag Products

Product Documents for S-Tag Epitope Tag Antibody (247) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for S-Tag Epitope Tag Antibody (247) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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