S-Tag Epitope Tag Antibody (G10-C8-R)
Novus Biologicals | Catalog # NBP3-32923
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Non-species specific
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # G10-C8-R expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide CKETAAAKFERQHMDS-KLH.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for S-Tag Epitope Tag Antibody (G10-C8-R)
Western Blot: S-Tag Epitope Tag Antibody (G10-C8-R) [NBP3-32923]
Western Blot: S-Tag Epitope Tag Antibody (G10-C8-R) [NBP3-32923] - Western blot analysis of S-Tag Epitope Tag on S tag recombinant protein with Mouse anti-S-Tag Epitope Tag antibody (NBP3-32923) at 1/1,000 dilution.Lysates/proteins at 50 ng/Lane.
Exposure time: 25 seconds; ECL;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32923) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for S-Tag Epitope Tag Antibody (G10-C8-R)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: S-Tag Epitope Tag
Alternate Names
KETAAAKFERQHMDS, S 7 Epitope tag
Additional S-Tag Epitope Tag Products
Product Documents for S-Tag Epitope Tag Antibody (G10-C8-R)
Certificate of Analysis
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Product Specific Notices for S-Tag Epitope Tag Antibody (G10-C8-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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