S100A8 293T Cell Transient Overexpression Lysate
Novus Biologicals | Catalog # H00006279-T01
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Key Product Details
Species
Human
Applications
Western Blot
Product Summary for S100A8 293T Cell Transient Overexpression Lysate
Quality control test: Transient overexpression cell lysate was tested with Anti-S100A8 antibody by Western Blots. Plasmid: pCMV-S100A8 full-length
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Product Specifications
Specificity
S100A8 293T Cell Transient Overexpression Lysate(Denatured)
Type
293T Cell Transient Overexpression
Protein State
Denatured
Scientific Data Images for S100A8 293T Cell Transient Overexpression Lysate
Other: S100A8 293T Cell Transient Overexpression Lysate [H00006279-T01]
S100A8 transfected lysate
Lane 1: S100A8 transfected lysate (10.34 KDa). Lane 2: Non-transfected lysate.
Formulation, Preparation, and Storage
Formulation
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Concentration
mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: S100A8
Long Name
S100 Calcium Binding Protein A8
Alternate Names
Calgranulin A, CFAG, MRP8
Gene Symbol
S100A8
Additional S100A8 Products
Product Documents for S100A8 293T Cell Transient Overexpression Lysate
Product Specific Notices for S100A8 293T Cell Transient Overexpression Lysate
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for S100A8 293T Cell Transient Overexpression Lysate
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Q: Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
A: Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link. -
Q: We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
A: Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
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