S100A9 Antibody Pair

Novus Biologicals | Catalog # H00006280-AP11

Novus Biologicals
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Key Product Details

Assay Type

Sandwich ELISA

Assay Range

0.03 ng/ml to 100 ng/ml

Reactivity

Human
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Product Specifications

Description

Quality control test: Standard curve using recombinant protein (H00006280-P01) as an analyte. This antibody pair set comes with matched antibody pair to detect and quantify protein level of human S100A9.

Conjugate

Unconjugated

Application Notes

Reagents are sufficient for at least 1-2 x 96 well plates using recommended protocols.

Scientific Data Images for S100A9 Antibody Pair

Sandwich ELISA: S100A9 Antibody Pair [H00006280-AP11] - Detection sensitivity ranging from 0.03 ng/ml to 100 ng/ml.

Kit Contents for S100A9 Antibody Pair

  • Capture antibody: rabbit MaxPab affinity purified polyclonal anti-S100A9 (100 ug)
  • Detection antibody: mouse monoclonal anti-S100A9 IgG1 Kappa (20 ug)

Preparation and Storage

Formulation

1x PBS, pH 7.4

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: S100A9

This antibody pair set comes with matched antibody pair to detect and quantify protein level of human S100A9.

Long Name

S100 Calcium Binding Protein A9

Alternate Names

Calgranulin B, MRP-14, Human S100A9 Capture Antibody

Gene Symbol

S100A9

Additional S100A9 Products

Product Documents for S100A9 Antibody Pair

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for S100A9 Antibody Pair

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Citations for S100A9 Antibody Pair

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FAQs for S100A9 Antibody Pair

Showing  1 - 2 of 2 FAQs Showing All
  • Q: Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?

    A: Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.

  • Q: We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.

    A:

    Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

  • Q: Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?

    A: Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.

  • Q: We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.

    A:

    Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

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