SA1 Antibody (1515) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43751

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Mouse (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1515

Format

Azide and BSA Free
View all SA1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SA1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (100%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

144 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SA1 Antibody (1515) - Azide and BSA Free

Immunoprecipitation: SA1 Antibody (1515) [NBP2-43751]

Immunoprecipitation: SA1 Antibody (1515) [NBP2-43751]

Immunoprecipitation: SA1 Antibody (1515) [NBP2-43751] - Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 ug of SA1 antibody [1515]. Western blot analysis was performed using SA1 antibody [1515] EasyBlot anti-Mouse IgG was used as a secondary reagent.
SA1 Antibody (1515)

Western Blot: SA1 Antibody (1515) [NBP2-43751] -

Western Blot: SA1 Antibody (1515) [NBP2-43751] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
SA1 Antibody (1515)

Western Blot: SA1 Antibody (1515) [NBP2-43751] -

Western Blot: SA1 Antibody (1515) [NBP2-43751] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
SA1 Antibody (1515) - Azide and BSA Free

Western Blot: SA1 Antibody (1515) [NBP2-43751] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:500.
SA1 Antibody (1515) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: SA1 Antibody (1515) [NBP2-43751] -

SA1 antibody [GT1515] detects SA1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SA1 protein stained by SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for SA1 Antibody (1515) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SA1

SA1 is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Alternate Names

cohesin subunit SA-1, nuclear protein stromal antigen 1, SA1, stromal antigen 1

Gene Symbol

STAG1

Additional SA1 Products

Product Documents for SA1 Antibody (1515) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SA1 Antibody (1515) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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