Salivary Amylase Alpha Antibody (2D4) - Azide and BSA Free

Novus Biologicals | Catalog # H00000276-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D4

Format

Azide and BSA Free
View all Salivary Amylase Alpha Primary Antibodies »

Product Specifications

Immunogen

AMY1A (NP_001008222, 172 a.a. ~ 245 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VRDCRLSGLLDLALGKDYVRSKIAEYMNHLIDIGVAGFRIDASKHMWPGDIKAILDKLHNLNSNWFPEGSKPFI

Specificity

AMY1A (2D4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Salivary Amylase Alpha Antibody (2D4) - Azide and BSA Free

Western Blot: Salivary Amylase Alpha Antibody (2D4) [H00000276-M04]

Western Blot: Salivary Amylase Alpha Antibody (2D4) [H00000276-M04]

Western Blot: Salivary Amylase Alpha Antibody (2D4) [H00000276-M04] - Analysis of AMY1A expression in human pancreas.
Immunohistochemistry-Paraffin: Salivary Amylase Alpha Antibody (2D4) [H00000276-M04]

Immunohistochemistry-Paraffin: Salivary Amylase Alpha Antibody (2D4) [H00000276-M04]

Immunohistochemistry-Paraffin: Salivary Amylase Alpha Antibody (2D4) [H00000276-M04] - Analysis of monoclonal antibody to AMY1A on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml
ELISA: Salivary Amylase Alpha Antibody (2D4) [H00000276-M04]

ELISA: Salivary Amylase Alpha Antibody (2D4) [H00000276-M04]

ELISA: Salivary Amylase Alpha Antibody (2D4) [H00000276-M04] - Detection limit for recombinant GST tagged AMY1A is 0.3 ng/ml as a capture antibody.

Applications for Salivary Amylase Alpha Antibody (2D4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Salivary Amylase Alpha

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein.

Alternate Names

1,4-alpha-D-glucan glucanohydrolase 1, alpha-amylase 1, AMY1, AMY1B, AMY1C, amylase, alpha 1A (salivary), amylase, alpha 1A; salivary, amylase, salivary, alpha-1A, EC 3.2.1.1, glycogenase, Salivary alpha-amylase, salivary amylase alpha 1A

Entrez Gene IDs

276 (Human); 11722 (Mouse)

Gene Symbol

AMY1A

OMIM

104700 (Human)

UniProt

NP_001008222 (Human)

Additional Salivary Amylase Alpha Products

Product Documents for Salivary Amylase Alpha Antibody (2D4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Salivary Amylase Alpha Antibody (2D4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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