SAP155 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35603

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SAP155 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SAP155 (NP_001005526.1).

Sequence:
MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGFYSAA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAP155 Antibody - BSA Free

SAP155 Antibody

Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP3-35603] -

Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP3-35603] - Immunofluorescence analysis of C6 using SAP155 Rabbit pAb at dilution of d100 (40x lens). Blue: DAPI for nuclear staining.
SAP155 Antibody

Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP3-35603] -

Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP3-35603] - Immunofluorescence analysis of L929 using SAP155 Rabbit pAb at dilution of d100 (40x lens). Blue: DAPI for nuclear staining.
SAP155 Antibody

Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP3-35603] -

Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP3-35603] - Immunofluorescence analysis of U-2OS using SAP155 Rabbit pAb at dilution of d100 (40x lens). Blue: DAPI for nuclear staining.
SAP155 Antibody

Western Blot: SAP155 Antibody [NBP3-35603] -

Western Blot: SAP155 Antibody [NBP3-35603] - Western Blot analysis of various lysates using SAP155 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for SAP155 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SAP155

SF3B155 is a subunit of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) that functions in pre-mRNA splicing. SF3B155 together with splicing factor 3a binds pre-mRNA upstream of the branchpoint sequence to anchor U2 snRNP to the pre-mRNA. SF3B155 is also a component of the U12-type splicesome that is involved in the splicing of a rare class of nuclear pre-mRNA introns.

Alternate Names

Hsh155, pre-mRNA processing 10, pre-mRNA splicing factor SF3b, 155 kDa subunit, Pre-mRNA-splicing factor SF3b 155 kDa subunit, Prp10, PRPF10, SAP 155, SAP155splicing factor 3b, subunit 1, 155kD, SF3B155, SF3b155PRP10, spliceosome associated protein 155, Spliceosome-associated protein 155, splicing factor 3B subunit 1, splicing factor 3b, subunit 1, 155kDa

Gene Symbol

SF3B1

Additional SAP155 Products

Product Documents for SAP155 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SAP155 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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