SARS-CoV-2 NSP10 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-07962

Novus Biologicals
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all SARS-CoV-2 NSP10 Primary Antibodies »

Product Specifications

Immunogen

Recombinant SARS-CoV-2 NSP10(4254A-S4393)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SARS-CoV-2 NSP10 Antibody - Azide and BSA Free

Western Blot: SARS-CoV-2 NSP10 AntibodyAzide and BSA Free [NBP3-07962]

Western Blot: SARS-CoV-2 NSP10 AntibodyAzide and BSA Free [NBP3-07962]

Western Blot: SARS-CoV-2 NSP10 Antibody [NBP3-07962] - Various lysates were subjected to SDS-PAGE followed by western blot with SARS-CoV-2 (2019-nCoV) NSP10 Protein antibody at dilution of 1:1000.

Applications for SARS-CoV-2 NSP10 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:4000-1:8000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 NSP10

SARS-CoV-2 Nonstructural Protein 10 (NSP10) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP10 is 139 amino acids (aa) with a theoretical molecular weight of 14.8 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP10 has 97.1% sequence identity and 99.3% sequence similarity (1). Functionally, NSP10 interacts with NSP14 which acts as an S-adenosylmethionine (SAM)-dependent (guanine-N7) methyl transferase (N7-MTase) and stimulates it by significantly increasing exoribonuclease activity (1,3,4). Additionally, SARS-CoV-2 NSP10 is proposed to be a cofactor for NSP16, which is a 2'-O-methyltransferase, and the interaction is needed for it to become active (1-4). NSP10 can interact with both NSP14 and NSP16 simultaneously, acting as both a cofactor for each as well as a molecular connector (4). A SARS-CoV-2 interactome study also revealed that NSP10 is involved in vesicle trafficking (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M.,... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Romano, M., Ruggiero, A., Squeglia, F., Maga, G., & Berisio, R. (2020). A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping. Cells. https://doi.org/10.3390/cells9051267

Alternate Names

GFL, Growth factor-like peptide, Non-structural protein 10

Gene Symbol

ORF1ab

Additional SARS-CoV-2 NSP10 Products

Product Documents for SARS-CoV-2 NSP10 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SARS-CoV-2 NSP10 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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