Q: Have you ever tested the specific activity of the recombnant enzyme NBP1-30198? Could it be used to acetylate substrate Spermine?

A: We have not tested the activity of NBP1-30198. This protein has only been validated as a positive control for SDS-PAGE and WB, so we would not recommend using it for any experiment requiring activity or functionality. I have checked our inventory to see if we have any SAT1 proteins that would be suitable for acetylating Spermine, but unfortunately we do not have any other SAT1 proteins.

Q: I need to radiolabel (iodinate) the SAT1 antibody. Which one should I use?

A: I would recommend NB110-41622 for you as it is a well validated antibody with very clean QC validation data, multiple applications/broad species reactivity. Additionally, this antibody has been cited by Liao et al. in The Journal of Biological Chemistry 2009;284(12):8174-8184 (https://www.jbc.org/article/S0021-9258(20)32529-1/fulltext).

Recombinant Human SAT1 His Protein

Novus Biologicals | Catalog # NBP1-30198

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all SAT1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-171 of Human SAT1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAKFVIRPAT AADCSDILRL IKELAKYEYM EEQVILTEKD LLEDGFGEHP FYHCLVAEVP KEHWTPEGHS IVGFAMYYFT YDPWIGKLLY LEDFFVMSDY RGFGIGSEIL KNLSQVAMRC RCSSMHFLVA EWNEPSINFY KRRGASDLSS EEGWRLFKID KEYLLKMATE E

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

22.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SAT1 His Protein

SDS-PAGE: Recombinant Human SAT1 His Protein [NBP1-30198]

SDS-Page: Recombinant Human SAT1 Protein [NBP1-30198] - Diamine N-acetyltransferase 1, 22.1 kDa (191aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation, and Storage

NBP1-30198

Formulation	20 mM Tris-HCl buffer (pH8.0), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAT1

Diamine N-acetyltransferase 1(or Spermidine/spermine-N1-acetyltransferase, SSAT1), as known as SAT-1, is a polyamine catabolic enzyme which catalyzes the acetylation of polyamines. This protein plays an important role in polyamine homoeostasis, since acetylated products are either excreted from the cell or oxidized by acetylpolyamine oxidase. A variety of other effects of increased SAT-1 activity include death of pancreatic cells, blockage of regenerative tissue growth, behavioral changes, keratosis follicularis spinulosa decalvans(KFSD), and hair loss. Recombinant human SAT-1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

DC21, diamine acetyltransferase 1, diamine N-acetyltransferase 1, EC 2.3.1.57, KFSD, Polyamine N-acetyltransferase 1, Putrescine acetyltransferase, SAT, Spermidine/spermine N(1)-acetyltransferase 1, spermidine/spermine N1-acetyltransferase, spermidine/spermine N1-acetyltransferase 1, spermidine/spermine N1-acetyltransferase alpha, SSAT-1, SSATKFSDX

Gene Symbol

SAT1

Additional SAT1 Products

Product Documents for Recombinant Human SAT1 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human SAT1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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