![Western Blot: SC35 Antibody [NBP1-79886]](https://resources.rndsystems.com/images/products/SC35-Antibody-Western-Blot-NBP1-79886-img0001.jpg "Western Blot: SC35 Antibody [NBP1-79886]")
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Format
Product Specifications
Immunogen
Marker
Clonality
Host
Isotype
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Scientific Data Images for SC35 Antibody - BSA Free
Western Blot: SC35 Antibody [NBP1-79886]
Western Blot: SC35 Antibody [NBP1-79886] - Hela Cell Lysate at a concentration of 1ug/ml in 12%gelApplications for SC35 Antibody - BSA Free
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: SC35
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional SC35 Products
Product Documents for SC35 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for SC35 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for SC35 Antibody - BSA Free
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Q: You do get 2 bands with this antibody - can you tell me which one is the correct one? I assume it is the top band, but do you know, what the protein in the bottom band is? We are interested in Co-Ip and are hence looking at high specificity in an SC-35 antibody.
A: SC35 has a predicted molecular weight of 25kDa, however, most of the antibodies detect bands in the range of 25-35kDa. In our QC data, this antibody (SC35 # NBP1-79886) was tested in HeLa Cell Lysate and showed two strong bands at 25 kDa and the other at 35 kDa range. Regarding your particular interest, SC35 antibody # NBP1-79886 has not been tested for Co-IP application. In fact, we have another antibody (SC-35 # NB100-1774) that we recommend for IP application and this antibody has Human specificity also.