Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SCAP Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human Sterol Regulatory Element Binding Protein Ceavage-Activating Protein using the numbering given in entry NP_036367.2 (GeneID 22937).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCAP Antibody - BSA Free

SCAP Antibody

Western Blot:SCAP Antibody [NBP2-04113] -

Western Blot:SCAP Antibody [NBP2-04113] -Whole cell lysate (10 ug) from HEK293T, Hep-G2, HeLa,Jurkat, and K-562 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-SCAP antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.
SCAP Antibody

Immunoprecipitation:SCAP Antibody [NBP2-04113] -

Immunoprecipitation:SCAP Antibody [NBP2-04113] -Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-SCAP antibody usedfor IP at 6 µg per reaction. SCAP was alsoimmunoprecipitated by a previous lot of this antibodyFor blotting immunoprecipitated SCAP,was used at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 30 seconds

Applications for SCAP Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Simple Western

1:25

Western Blot

1:2000-1:10000
Application Notes

See Simple Western Antibody Database for Simple Western validation: Tested in Caco-2, separated by Size, antibody dilution of 1:25

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SCAP

Sterol regulatory element binding protein (SREBP) cleavage-activating protein (SCAP) is a molecular chaperone that plays a role in the biosynthesis of cholesterol and triglycerides (1). SCAP is a membrane protein that helps shuttle SREBP from the endoplasmic reticulum (ER) via coat protein II (COPII) vesicles to the golgi where SREBP is cleaved via protease S1P and S2P, becomes activated, and is translocated to the nucleus for transcription (1-3). The SCAP protein has a theoretical molecular weight of 140 kDa and is comprised of 1276 amino acids with an N-terminal domain containing 8 transmembrane alpha helices, where transmembrane 2-6 consist of a sterol-sensing domain, and a C-terminal domain with WD40 motif repeats (1,3). The SCAP-SREBP association plays a pivotal role in regulating lipid metabolism and lipogenesis (1,4). SREBP activation via SCAP is associated with accumulation of triglycerides and cholesterol leading to metabolic disorders including diabetes, atherosclerosis, and nonalcoholic fatty liver disease (NAFLD) (1,4). Animal models have revealed that inhibition or blocking of SCAP using antagonists may be a potential therapeutic target for hyperlipidemia and hypertriglyceridemia (1,4).

References

1. Lee, S. H., Lee, J. H., & Im, S. S. (2020). The cellular function of SCAP in metabolic signaling. Experimental & molecular medicine, 52(5), 724-729. https://doi.org/10.1038/s12276-020-0430-0

2. Cheng, X., Li, J., & Guo, D. (2018). SCAP/SREBPs are Central Players in Lipid Metabolism and Novel Metabolic Targets in Cancer Therapy. Current topics in medicinal chemistry, 18(6), 484-493. https://doi.org/10.2174/1568026618666180523104541

3. Brown, M. S., Radhakrishnan, A., & Goldstein, J. L. (2018). Retrospective on Cholesterol Homeostasis: The Central Role of Scap. Annual review of biochemistry, 87, 783-807. https://doi.org/10.1146/annurev-biochem-062917-011852

4. Moon Y. A. (2017). The SCAP/SREBP Pathway: A Mediator of Hepatic Steatosis. Endocrinology and metabolism (Seoul, Korea), 32(1), 6-10. https://doi.org/10.3803/EnM.2017.32.1.6

Alternate Names

KIAA0199, PSEC0227, SREBF chaperone, SREBP cleavage-activating protein, sterol regulatory element binding protein cleavage-activating protein

Entrez Gene IDs

22937 (Human)

Gene Symbol

SCAP

UniProt

NP_036367.2 (Human)

Additional SCAP Products

Product Documents for SCAP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SCAP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SCAP Antibody - BSA Free

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