SCD-1 Antibody (A9A11-R)

Novus Biologicals | Catalog # NBP3-32932

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # A9A11-R expressed in HEK293
View all SCD-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human SCD-1 aa 310-509 / 509. (Uniprot: O00767)

Localization

Endoplasmic reticulum membrane.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SCD-1 Antibody (A9A11-R)

SCD-1 Antibody (A9A11-R)

Western Blot: SCD-1 Antibody (A9A11-R) [NBP3-32932]

Western Blot: SCD-1 Antibody (A9A11-R) [NBP3-32932] - Western blot analysis of SCD-1 on different lysates with Mouse anti-SCD-1 antibody (NBP3-32932) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: SK-MEL-28 cell lysate
Lane 5: A375 cell lysate
Lane 6: A431 cell lysate
Lane 7: A549 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 42 kDa
Observed band size: 37 kDa

Exposure time: 3 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32932) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for SCD-1 Antibody (A9A11-R)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCD-1

SCD-1 encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015]

Long Name

Stearoyl CoA-desaturase 1, Isoform of the Delta9 Desaturase

Alternate Names

SCD1

Gene Symbol

SCD

Additional SCD-1 Products

Product Documents for SCD-1 Antibody (A9A11-R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SCD-1 Antibody (A9A11-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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