SCOTfluor glucose probe 510

Tocris Bioscience | Catalog # 7447

Fluorescent glucose probe
Tocris Bioscience
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Key Product Details

Description

Fluorescent glucose probe

Wavelength

Excitation = 490 nm, Emission = 605 nm

Product Description

SCOTfluor glucose probe 510 is a fluorescent probe for visualizing glucose uptake in vivo. It can be multiplexed with BFP and GFP. Excitation and emission maxima (λ) are 490 and 605 nm, respectively.

Scientific Data Images for SCOTfluor glucose probe 510

Application of SCOTfluor glucose probe 510 in HeLa cells

Application of SCOTfluor glucose probe 510 in HeLa cells.

Fluorescence microscopy image of GLUT4-EGFP HeLa cells treated with SCOTfluor glucose probe 510 (100 μm). Colors: green (GLUT4-EGFP), red (SCOTfluor glucose probe 510), blue: Hoechst 33342 (Catalog # 5117). Image kindly provided by Prof. Marc Vendrell, acquired by Dr Sam Benson, Centre for Inflammation Research, University of Edinburgh.

Application of SCOTfluor glucose probe 510 in zebrafish embryos

Application of SCOTfluor glucose probe 510 in zebrafish embryos.

In vivo images of zebrafish embryos (28 h post fertilization) after injection of  SCOTfluor glucose probe 510 (50 pmol) to the yolk sac in wildtype zebrafish (left) or zebrafish injected at one cell stage with 4.2 ng anti-sense glu2 morpholino (right). Image kindly provided by Prof. Marc Vendrell, acquired by Dr Yi Feng, Centre for Inflammation Research, University of Edinburgh.

Licensing Information

Sold under license from the University of Edinburgh

Optical Data

SCOTfluor glucose probe 510 Dye Spectra
Emission Color Orange
λabs 490 nm
λem 605 nm
Closest Laser line 488 nm
Cell Permeable Yes
Application Fluorescence Microscopy, Confocal Microscopy, in vivo Imaging

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Product Specifications for SCOTfluor glucose probe 510

Molecular Weight

405.23

Formula

C12H14N4O7Se

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

2-Deoxy-2-[(7-nitro-2,1,3-benzoselenadiazol-4-yl)amino]-D-glucose

CAS Number

2490298-72-9

PubChem ID

155694629

InChI Key

LXWDEVJSKWMYFZ-BGCUHRRXSA-N

SMILES

OC[C@H]1OC([C@@H]([C@H]([C@@H]1O)O)NC2=CC=C(N(=O)=O)C3=N[Se]N=C23)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 4.05 10

Preparing Stock Solutions for SCOTfluor glucose probe 510

The following data is based on the product molecular weight 405.23.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 24.68 mL 123.39 mL 246.77 mL
0.5 mM 4.94 mL 24.68 mL 49.35 mL
1 mM 2.47 mL 12.34 mL 24.68 mL
5 mM 0.49 mL 2.47 mL 4.94 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.

Product Documents for SCOTfluor glucose probe 510

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SCOTfluor glucose probe 510

For research use only

Citations for SCOTfluor glucose probe 510

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