SDHA Antibody (0Y8X5)

Novus Biologicals | Catalog # NBP3-15473

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0Y8X5 expressed in HEK293
View all SDHA Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 550-650 of human SDHA (P31040). KTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SDHA Antibody (0Y8X5)

Western Blot: SDHA Antibody (0Y8X5) [NBP3-15473]

Western Blot: SDHA Antibody (0Y8X5) [NBP3-15473]

Western Blot: SDHA Antibody (0Y8X5) [NBP3-15473] - Analysis of extracts of various cell lines, using SDHA Rabbit mAb (NBP3-15473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
SDHA Antibody (0Y8X5)

Immunocytochemistry/ Immunofluorescence: SDHA Antibody (0Y8X5) [SDHA] -

Immunocytochemistry/ Immunofluorescence: SDHA Antibody (0Y8X5) [SDHA] - Confocal imaging of paraffin-embedded Human colon cancer tissue using SDHA Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
SDHA Antibody (0Y8X5)

Immunocytochemistry/ Immunofluorescence: SDHA Antibody (0Y8X5) [SDHA] -

Immunocytochemistry/ Immunofluorescence: SDHA Antibody (0Y8X5) [SDHA] - Confocal imaging of HeLa cells using SDHA Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for SDHA Antibody (0Y8X5)

Application
Recommended Usage

Western Blot

1:500 -1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SDHA

The SDHA gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations

Long Name

SDHA

Alternate Names

CMD1GG, MC2DN1, NDAXOA, PGL5, PPGL5, SDH1, SDH2, SDHF

Gene Symbol

SDHA

Additional SDHA Products

Product Documents for SDHA Antibody (0Y8X5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SDHA Antibody (0Y8X5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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