SDHB Antibody (SDHB/2382) [Alexa Fluor™ Plus 488]

Novus Biologicals | Catalog # NBP3-08537AFP488

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Protein Array

Label

Alexa Fluor Plus 488

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SDHB/2382
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Product Specifications

Immunogen

Recombinant fragment (around aa 165-273) of human SDHB protein (exact sequence is proprietary) (Uniprot: P21912)

Localization

Cytoplasmic (Mitochondria)

Marker

Pheochromocytoma Marker

Specificity

Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation. Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Applications for SDHB Antibody (SDHB/2382) [Alexa Fluor™ Plus 488]

Application
Recommended Usage

Protein Array

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SDHB

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Alternate Names

EC 1.3.5.1, FLJ92337, Ip, Iron-sulfur subunit of complex II, PGL4, SDH, SDH1, SDH2, SDHIP, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Gene Symbol

SDHB

Additional SDHB Products

Product Documents for SDHB Antibody (SDHB/2382) [Alexa Fluor™ Plus 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SDHB Antibody (SDHB/2382) [Alexa Fluor™ Plus 488]



This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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