Semaphorin 3E Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-23381

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Semaphorin 3E Primary Antibodies »

Product Specifications

Immunogen

Semaphorin 3E (N-terminal region) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminal region of human Semaphorin 3E. This sequence is well conserved in rat, mouse, and chicken Semaphorin 3E, and has low homology to other semaphorin family members. [UniProt# O15041]

Specificity

The antibody detects 87 and 61 kDa bands corresponding to the full-length and furin-proccessed forms of Semaphorin 3E in human PC-3 and rat PC12 cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for Semaphorin 3E Antibody - BSA Free

Semaphorin 3E Antibody

Western Blot: Semaphorin 3E Antibody [NBP3-23381] -

Western Blot: Semaphorin 3E Antibody [NBP3-23381] - Western blot analysis of rat PC12 cells (lanes 1 & 2) and human PC-3 cells (lanes 3 & 4). The blots were probed with anti-Semaphorin 3E (N-terminal region) antibody in absence (lanes 1 & 3) or presence of Semaphorin 3E (N-terminal region) blocking peptide (lanes 2 & 4).

Applications for Semaphorin 3E Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Semaphorin 3E

Semaphorins, such as Semaphorin 3E, are characterized by a conserved domain of about 500 amino acids. These proteins are involved in embryonic development, and some behave as neural guidance molecules.

Alternate Names

Sema3E, SEMAH

Gene Symbol

SEMA3E

Additional Semaphorin 3E Products

Product Documents for Semaphorin 3E Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Semaphorin 3E Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Proteoglycans

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