Semaphorin 4D/CD100 Antibody - Humanized, IgG4SP, (Research Grade pepinemab Biosimilar) - Low Endotoxin, Azide and BSA Free

Novus Biologicals | Catalog # NBP3-28669

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG4 expressed in CHO

Format

Low Endotoxin, Azide and BSA Free
View all Semaphorin 4D/CD100 Primary Antibodies »

Product Specifications

Immunogen

SEMA4D / CD100

Clonality

Monoclonal

Host

Human

Isotype

IgG4

Description

The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Also known as 'pepinemab'.

IgG4SP is human IgG4 with S228P mutation.

This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Scientific Data Images

Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP

In vivo assay: Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP[NBP3-28669] -

Pepinemab inhibited the tumor growth of CT26 on balb/c mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 76.9% at 50 mpk at D24.
Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP

ELISA: Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP[NBP3-28669] -

Immobilized human SEMA4D/CD100, His Tag at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP, EC50=0.0081 ug/mL.
Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP

Flow Cytometry: Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP[NBP3-28669] -

Human C100 HEK293 cells were stained with Semaphorin 4D/CD100 Antibody (pepinemab) - Humanized, IgG4SP and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.3227 nM.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 4D/CD100

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Alternate Names

A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ

Gene Symbol

SEMA4D

UniProt

Q92854

Additional Semaphorin 4D/CD100 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Proteoglycans

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Protocols

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