Senataxin Antibody (BLR050F) - BSA Free

Novus Biologicals | Catalog # NBP3-14676

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR050F

Format

BSA Free
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Product Specifications

Immunogen

between 900 and 950

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Senataxin Antibody (BLR050F) - BSA Free

Western Blot: Senataxin Antibody (BLR050F) [NBP3-14676]

Western Blot: Senataxin Antibody (BLR050F) [NBP3-14676]

Western Blot: Senataxin Antibody [NBP3-14676] - Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7,Hep-G2, A-549, SW620, SK-MEL-28, Jurkat, K-562,RMPI-8226, and 786-O cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-Senataxin recombinantmonoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 30 seconds.
Immunoprecipitation: Senataxin Antibody (BLR050F) [NBP3-14676]

Immunoprecipitation: Senataxin Antibody (BLR050F) [NBP3-14676]

Immunoprecipitation: Senataxin Antibody [NBP3-14676] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-Senataxinrecombinant monoclonal antibody used for IP at 6 ul per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibodies. For blottingimmunoprecipitated Senataxin, was used at1:1000. Chemiluminescence with an exposure time of 10seconds.
Senataxin Antibody (BLR050F)

Flow Cytometry:Senataxin Antibody [NBP3-14676] -

Flow Cytometry:Senataxin Antibody [NBP3-14676] -: Rabbit anti-Senataxinrecombinant monoclonal [BLR050F] or isotypecontrol (unshaded). Secondary: DyLight® 488-conjugatedgoat anti-rabbit IgG

Applications for Senataxin Antibody (BLR050F) - BSA Free

Application
Recommended Usage

Flow Cytometry

Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl

Immunoprecipitation

6 µl/1 mg lysate

Western Blot

1:1000

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Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with, BSA Free

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Senataxin

Mutations in Senataxin result in the neurodegenerative disorders Ataxia-Oculomotor Apraxia 2 (AOA2) and amyotrophic lateral sclerosis (ALS4). Senataxin bears an RNA/DNA helicase domain suggesting a role in DNA and RNA processing and has recently been found to be involved in the DNA damage response. Alternate names for Senataxin include SEN1 homolog, SETX, ALS4, KIAA0625, SCAR1, and AOA2.

Alternate Names

ALS4recessive, non-Friedreich type 1, AOA2FLJ43459, DKFZp781B151, KIAA0625FLJ12840, senataxin, SETX

Gene Symbol

SETX

Additional Senataxin Products

Product Documents for Senataxin Antibody (BLR050F) - BSA Free

Certificate of Analysis

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Product Specific Notices for Senataxin Antibody (BLR050F) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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