Septin-2 Antibody
Novus Biologicals | Catalog # NBP1-06076
![Western Blot: Septin-2 Antibody [NBP1-06076]](https://resources.rndsystems.com/images/products/Septin-2-Antibody-Western-Blot-NBP1-06076-img0002.jpg "Western Blot: Septin-2 Antibody [NBP1-06076]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence SKQQPTQFINPET-C corresponding to N-Terminus according to NP_001008491.1.
Epitope
SKQQPTQFINPET-C
Specificity
Reported variants represent identical protein: NP_001008492.1, NP_006146.1, NP_004395.1, NP_001008491.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Septin-2 Antibody
Western Blot: Septin-2 Antibody [NBP1-06076]
Western Blot: Septin-2 Antibody [NBP1-06076] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076]](https://resources.rndsystems.com/images/products/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0005.jpg "Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076]")
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076]
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Cortex. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076]](https://resources.rndsystems.com/images/products/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0003.jpg "Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076]")
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076]
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Breast tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076]](https://resources.rndsystems.com/images/products/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0004.jpg "Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076]")
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076]
Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Tonsil tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Septin-2 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
2.5 ug/mL
Peptide ELISA
Detection limit 1:32000
Western Blot
0.05 - 0.2 ug/mL
Application Notes
Western blot: Approx. 40kDa band observed in Human Testis lysates (calculated MW of 41.5 kDa according to NP_001008491.1). Primary incubation was 1 hour.
IHC: Paraffin embedded Human Breast, Tonsil and Brain (Cortex).
IHC: Paraffin embedded Human Breast, Tonsil and Brain (Cortex).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Septin-2
Alternate Names
DIFF6NEDD-5, hNedd5, KIAA0158Neural precursor cell expressed developmentally down-regulated protein 5, NEDD5Pnutl3, neural precursor cell expressed, developmentally down-regulated 5, septin 2, septin-2
Gene Symbol
SEPTIN2
UniProt
Additional Septin-2 Products
Product Documents for Septin-2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Septin-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Septin-2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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