SERCA2 ATPase Antibody (10F0M9)

Name: SERCA2 ATPase Antibody (10F0M9)
Brand: Novus Biologicals
SKU: NBP3-15429
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15429

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10F0M9 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 943-1042 of human SERCA2 ATPase (P16615). HFLILYVEPLPLIFQITPLNVTQWLMVLKISLPVILMDETLKFVARNYLEPGKECVQPATKSCSFSACTDGISWPFVLLIMPLVIWVYSTDTNFSDMFWS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SERCA2 ATPase Antibody (10F0M9)

Western Blot: SERCA2 ATPase Antibody (10F0M9) [NBP3-15429]

Western Blot: SERCA2 ATPase Antibody (10F0M9) [NBP3-15429] - Western blot analysis of extracts of various cell lines, using SERCA2 ATPase Rabbit mAb (NBP3-15429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation: SERCA2 ATPase Antibody (10F0M9) [NBP3-15429]

Immunoprecipitation: SERCA2 ATPase Antibody (10F0M9) [NBP3-15429] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SERCA2 ATPase antibody (NBP3-15429). Western blot was performed from the immunoprecipitate using SERCA2 ATPase antibody (NBP3-15429) at a dilition of 1:3000.

Western Blot: SERCA2 ATPase Antibody (10F0M9) [SERCA2 ATPase] -

Western Blot: SERCA2 ATPase Antibody (10F0M9) [SERCA2 ATPase] - Western blot analysis of various lysates using SERCA2 ATPase Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (10F0M9) [SERCA2 ATPase] -

Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (10F0M9) [SERCA2 ATPase] - Confocal imaging of Hep G2 cells using SERCA2 ATPase Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

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Applications for SERCA2 ATPase Antibody (10F0M9)

Application

Recommended Usage

Immunoprecipitation

1:1000 - 1:5000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SERCA2 ATPase

Sarcoplasmic/endoplasmic reticulum Calcium-ATPase 2 (SERCA2) is a magnesium-dependent pump that catalyzes the hydrolysis of ATP and translocates calcium from the cytosol to the sarcoplasmic reticulum lumen. There are 2 SERCA2 isoforms that differ in the 3' UTR. The SERCA2A isoform is highly expressed in heart and slow twitch skeletal muscle and is important to the regulation of the contraction and relaxation cycle, while the SERCA2B isoform is expressed in smooth muscle and adult epidermis. SERCA2 is also known as ATP2A2, ATP2B, DAR, DD, and MGC45367.

Long Name

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2

Alternate Names

ATP2A2, ATP2B, Calcium pump 2, SERCA2, SR Ca(2+)-ATPase 2

Gene Symbol

ATP2A2

Additional SERCA2 ATPase Products

Product Documents for SERCA2 ATPase Antibody (10F0M9)

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Certificate of Analysis

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Product Specific Notices for SERCA2 ATPase Antibody (10F0M9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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