Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74113

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI8D5

Format

Azide and BSA Free
View all Serpin C1/Antithrombin-III Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SERPINC1 (NP_000479) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

52.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free

Western Blot: Serpin C1/Antithrombin-III Antibody (OTI8D5)Azide and BSA Free [NBP2-74113]

Western Blot: Serpin C1/Antithrombin-III Antibody (OTI8D5)Azide and BSA Free [NBP2-74113]

Western Blot: Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free [NBP2-74113] - Analysis of extracts (10ug) from 10 Human tissue by using anti-SERPINC1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Flow Cytometry: Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free [NBP2-74113]

Flow Cytometry: Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free [NBP2-74113]

Flow Cytometry: Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free [NBP2-74113] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Antithrombin III antibody, and then analyzed by flow cytometry.

Applications for Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin C1/Antithrombin-III

Antithrombin III (ATH III) is a plasma protein synthesised in the liver. Normal plasma levels are 115 to 160mg/L. ATH III consists of a single polypeptide chain and has a molecular weight of 58kDa. ATH III is a serine protease inhibitor and regulates coagulation by the formation of covalently liked complexes. ATH III also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000 fold. Low levels of ATH III (functional or concentration) are associated with deep vein thrombosis and pulmonary embolism. Antithrombin assays are based on immunologic, enzymatic, or clotting properties

Alternate Names

Antithrombin-III, ATIII

Gene Symbol

SERPINC1

Additional Serpin C1/Antithrombin-III Products

Product Documents for Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Serpin C1/Antithrombin-III Antibody (OTI8D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail