SF3A3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-94076
![Western Blot: SF3A3 AntibodyAzide and BSA Free [NBP2-94076]](https://resources.rndsystems.com/images/products/SF3A3-Antibody-Western-Blot-NBP2-94076-img0008.jpg "Western Blot: SF3A3 AntibodyAzide and BSA Free [NBP2-94076]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 292-501 of human SF3A3 (NP_006793.1). SLESLDTSLFAKNPKSKGTKRDTERNKDIAFLEAQIYEYVEILGEQRHLTHENVQRKQARTGEEREEEEEEQISESESEDEENEIIYNPKNLPLGWDGKPIPYWLYKLHGLNINYNCEICGNYTYRGPKAFQRHFAEWRHAHGMRCLGIPNTAHFANVTQIEDAVSLWAKLKLQKASERWQPDTEEEYEDSSGNVVNKKTYEDLKRQGLL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SF3A3 Antibody - BSA Free
Western Blot: SF3A3 AntibodyAzide and BSA Free [NBP2-94076]
Western Blot: SF3A3 Antibody [NBP2-94076] - Western blot analysis of extracts of various cell lines, using SF3A3 antibody (NBP2-94076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunocytochemistry/ Immunofluorescence: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]](https://resources.rndsystems.com/images/products/SF3A3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94076-img0001.jpg "Immunocytochemistry/ Immunofluorescence: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]")
Immunocytochemistry/ Immunofluorescence: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]
Immunocytochemistry/Immunofluorescence: SF3A3 Antibody [NBP2-94076] - Analysis of U2OS cells using SF3A3 at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]](https://resources.rndsystems.com/images/products/SF3A3-Antibody-Immunohistochemistry-Paraffin-NBP2-94076-img0007.jpg "Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]")
Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]
Immunohistochemistry-Paraffin: SF3A3 Antibody [NBP2-94076] - Immunohistochemistry of paraffin-embedded mouse kidney using SF3A3 Rabbit pAb (NBP2-94076) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Western Blot: SF3A3 AntibodyAzide and BSA Free [NBP2-94076]](https://resources.rndsystems.com/images/products/SF3A3 Antibody-Western Blot-NBP2-94076-img0009.jpg "Western Blot: SF3A3 AntibodyAzide and BSA Free [NBP2-94076]")
Western Blot: SF3A3 AntibodyAzide and BSA Free [NBP2-94076]
Western Blot: SF3A3 Antibody [NBP2-94076] - Western blot analysis of extracts of various cell lines, using SF3A3 antibody (NBP2-94076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.![Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]](https://resources.rndsystems.com/images/products/SF3A3-Antibody-Immunohistochemistry-Paraffin-NBP2-94076-img0002.jpg "Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]")
Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]
Immunohistochemistry-Paraffin: SF3A3 Antibody [NBP2-94076] - Immunohistochemistry of paraffin-embedded rat ovary using SF3A3 Rabbit pAb (NBP2-94076) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]](https://resources.rndsystems.com/images/products/SF3A3-Antibody-Immunohistochemistry-Paraffin-NBP2-94076-img0003.jpg "Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]")
Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]
Immunohistochemistry-Paraffin: SF3A3 Antibody [NBP2-94076] - Immunohistochemistry of paraffin-embedded rat brain using SF3A3 Rabbit pAb (NBP2-94076) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]](https://resources.rndsystems.com/images/products/SF3A3-Antibody-Immunohistochemistry-Paraffin-NBP2-94076-img0004.jpg "Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]")
Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]
Immunohistochemistry-Paraffin: SF3A3 Antibody [NBP2-94076] - Immunohistochemistry of paraffin-embedded human colon using SF3A3 Rabbit pAb (NBP2-94076) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]](https://resources.rndsystems.com/images/products/SF3A3-Antibody-Immunohistochemistry-Paraffin-NBP2-94076-img0005.jpg "Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]")
Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]
Immunohistochemistry-Paraffin: SF3A3 Antibody [NBP2-94076] - Immunohistochemistry of paraffin-embedded human esophageal cancer using SF3A3 Rabbit pAb (NBP2-94076) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]](https://resources.rndsystems.com/images/products/SF3A3-Antibody-Immunohistochemistry-Paraffin-NBP2-94076-img0006.jpg "Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]")
Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]
Immunohistochemistry-Paraffin: SF3A3 Antibody [NBP2-94076] - Immunohistochemistry of paraffin-embedded mouse testis using SF3A3 Rabbit pAb (NBP2-94076) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Applications for SF3A3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SF3A3
Alternate Names
PRP9, PRPF9, SAP 61, SAP61splicing factor 3a, subunit 3, 60kD, SF3a60pre-mRNA splicing factor SF3a (60kD), spliceosome associated protein 61, Spliceosome-associated protein 61, splicing factor 3A subunit 3, splicing factor 3a, subunit 3, 60kDa
Gene Symbol
SF3A3
Additional SF3A3 Products
Product Documents for SF3A3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SF3A3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.govCustomer Reviews for SF3A3 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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