SGSM3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-60726
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
between 699 and 749
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SGSM3 Antibody - BSA Free
Western Blot: SGSM3 Antibody [NBP2-60726]
Western Blot: SGSM3 Antibody [NBP2-60726] - Detection of human and mouse SGSM3 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-SGSM3 antibody NBP2-60726 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.Western Blot: SGSM3 Antibody [NBP2-60726]
Western Blot: SGSM3 Antibody [NBP2-60726] - Detection of human and mouse SGSM3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SGSM3 antibody NBP2-60726 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: SGSM3 Antibody [NBP2-60726]
Immunoprecipitation: SGSM3 Antibody [NBP2-60726] - Detection of human SGSM3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-SGSM3 antibody NBP2-60726 (lot NBP2-60726-2) used for IP at 20 ul/mg lysate. SGSM3 was also immunoprecipitated by a previous lot of this antibody (lot NBP2-60726-1) and another rabbit anti-SGSM3 antibody. For blotting immunoprecipitated SGSM3, NBP2-60726 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.Applications for SGSM3 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
8-15 ul/mg lysate
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-buffered Saline containing 0.1% BSA
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4°C.
Background: SGSM3
Alternate Names
DJ1042K10.2, DKFZp761D051, MAP, merlin binding protein, Merlin-associated protein, RABGAPLP, Rab-GTPase-activating protein-like protein, RUN and SH3 containing 3, RUN and TBC1 domain containing 3, RUN and TBC1 domain-containing protein 3, RUSC3, RUTBC3, small G protein signaling modulator 3, small G protein signaling modulator 3 protein
Gene Symbol
SGSM3
Additional SGSM3 Products
Product Documents for SGSM3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SGSM3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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