Sheep IgG VisUCyte HRP Polymer Antibody

VC006 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
VC006-025
VC006-050
VC006-125
VC006-250

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Detection of Progesterone R/NR3C3 in Human Breast Cancer Tissue.

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Sheep IgG VisUCyte HRP Polymer Antibody Summary

Species Reactivity

Sheep

Specificity

Detects heavy ( gamma ) chains on sheep IgG and light chains on all sheep immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin sheep serum proteins or IgG from human, mouse or rabbit, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.

Source

Polyclonal Donkey IgG

Purification

Antigen Affinity-purified

Immunogen

Sheep IgG

Formulation

Supplied as a solution in PBS with preservative.

Label

HRP Polymer

Applications

Recommended Concentration

Sample

Immunohistochemistry

Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

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Detection of Progesterone R/NR3C3 in Human Breast Cancer Tissue. Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) with Anti-Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color). View our protocol for the VisUCyte HRP Polymer Detection Reagent.

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name

Immunoglobulin G

Alternate Names

IgG; immunoglobulin heavy constant gamma 1 (G1m marker)