SHIP Antibody (SHIP-02) - BSA Free

Novus Biologicals | Catalog # NBP1-45077

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow (Intracellular), CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # SHIP-02

Format

BSA Free
View all SHIP Primary Antibodies »

Product Specifications

Immunogen

Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.

Reactivity Notes

Cross reacts with Human.

Specificity

The antibody SHIP-02 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SHIP Antibody (SHIP-02) - BSA Free

SHIP Antibody (SHIP-02) - BSA Free

Flow Cytometry: Mouse Monoclonal SHIP Antibody (SHIP-02) [NBP1-45077] -

Flow Cytometry: Mouse Monoclonal SHIP Antibody (SHIP-02) [NBP1-45077] - Separation of MOLT-4 cells stained using anti-SHIP1 (SHIP-02) purified antibody (concentration in sample 3 μg/ml, GAM APC, red-filled) from MOLT-4 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
Western Blot: SHIP Antibody (SHIP-02)BSA Free [NBP1-45077]

Western Blot: SHIP Antibody (SHIP-02)BSA Free [NBP1-45077]

Western Blot: SHIP Antibody (SHIP-02) [NBP1-45077] - Analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-04) Lane 2,3: immunostaining with anti-human SHIP-1 (SHIP-01; cat. no. NB110-81538) Lane 4,5: immunostaining with anti-human SHIP-1 (SHIP-02)

Applications for SHIP Antibody (SHIP-02) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

2-5 ug/ml

Western Blot

1:1000
Application Notes
Western Blotting - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. Application note:. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SHIP

SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5'inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.

Long Name

SH2-containing Inositol Phosphatase

Alternate Names

INPP5D

Gene Symbol

INPP5D

Additional SHIP Products

Product Documents for SHIP Antibody (SHIP-02) - BSA Free

Certificate of Analysis

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Product Specific Notices for SHIP Antibody (SHIP-02) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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