SIAH1/2 Antibody (8G7H12) - BSA Free

Novus Biologicals | Catalog # NB110-93596

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Porcine, Drosophila, Mouse (Negative), Zebrafish

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8G7H12

Format

BSA Free
View all SIAH1/2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a C-terminal portion of the drosophila SINA protein (within residues 280-331). [Swiss-Prot# P21461]

Reactivity Notes

Does not react with mouse.

Localization

Nuclear, Cytoplasmic

Specificity

This is specific for human SIAH 1 and 2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse SIAH1/2 Antibody (8G7H12) - BSA Free (NB110-93596) is a monoclonal antibody validated for use in WB and IP. Anti-SIAH1/2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SIAH1/2 Antibody (8G7H12) - BSA Free

Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596]

Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596]

Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly speci

Applications for SIAH1/2 Antibody (8G7H12) - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
This SIAH1/2 antibody is useful for Immunoprecipitation and Western blot, where a band is seen at ~37 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SIAH1/2

The Drosophila SINA (seven in absentia) protein is an E3 ubiquitin ligase component of the RAS signal transduction pathway. The RAS signal pathway controls cell proliferation, differentiation, and survival, and regulation of this pathway is critical for normal development. In Drosophila SINA serves as a downstream gatekeeper required for proper RAS signal transduction. Similarly to SINA in Drosophila, the human protein SIAH has also been shown to be required for oncogenic RAS signaling in cancer.

Alternate Names

E3 ubiquitin-protein ligase SIAH1, EC 6.3.2, EC 6.3.2.-, FLJ08065, hSIAH1, HUMSIAH, Seven in absentia homolog 1, seven in absentia homolog 1 (Drosophila), Siah-1, SIAH1A, siah-1a

Entrez Gene IDs

6478 (Human); 6477 (Human)

Gene Symbol

SIAH1

UniProt

NP_005058.2 (Human)

Additional SIAH1/2 Products

Product Documents for SIAH1/2 Antibody (8G7H12) - BSA Free

Certificate of Analysis

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Product Specific Notices for SIAH1/2 Antibody (8G7H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SIAH1/2 Antibody (8G7H12) - BSA Free

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