Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-75189

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Cynomolgus Monkey, Rhesus Macaque

Applications

Block/Neutralize, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # hL22 (Epratuzumab)

Format

Azide and BSA Free
View all Siglec-2/CD22 Primary Antibodies »

Product Specifications

Immunogen

This antibody was prepared by the humanization of LL2 (EPB-2), a murine anti-CD22 IgG2a raised against Raji Burkitt lymphoma cells. Murine sequences comprise 5-10% of the molecule, with the remainder being human framework sequences, which greatly reduces the potential for immunogenicity (Traczewski, 2010).

Specificity

This antibody is specific for the 3rd Ig-like domain of human CD22 (epitope B), a cell surface glycoprotein present on mature B-cells and on many types of malignant B-cells.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images

Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric - Azide and BSA Free [NBP2-75189]

Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric - Azide and BSA Free [NBP2-75189]

Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Rhesus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric - Azide and BSA Free [NBP2-75189]

Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric - Azide and BSA Free [NBP2-75189]

Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Eptratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric - Azide and BSA Free [NBP2-75189]

Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric - Azide and BSA Free [NBP2-75189]

Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Cynomolgus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications

Application
Recommended Usage

Flow Cytometry

1:100 - 1:1000

Immunoprecipitation

1:10 - 1:500
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Siglec-2/CD22

CD22 is a single-pass type 1 membrane glycoprotein found in the cytoplasm and cell membrane of B-lymphocytes. CD22 has at least two isoforms (alpha and beta) produced by alternate splicing. The protein exists predominantly as a monomer of the beta isoform (95 kDa). It is also found as a heterodimer of the beta and alpha isoforms.

Long Name

Sialic Acid Binding Ig-like Lectin 2

Alternate Names

BL-CAM, CD22, Siglec2

Gene Symbol

CD22

Additional Siglec-2/CD22 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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  • Q: I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?

    A: We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.

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