Siglec-3/CD33 Antibody (HIM3-4) - BSA Free

Novus Biologicals | Catalog # NB500-508

Clone HIM3-4 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Primate

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HIM3-4

Format

BSA Free
View all Siglec-3/CD33 Primary Antibodies »

Product Specifications

Immunogen

NFMY-9s human cell line

Localization

Cell Membrane

Specificity

The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. HLDA V; WS Code M MA112 HLDA VI; WS Code M MA47

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Siglec-3/CD33 Antibody (HIM3-4) - BSA Free

Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) - BSA Free [NB500-508]

Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) - BSA Free [NB500-508]

Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) [NB500-508] - Using the Biotin direct conjugate Surface staining of human peripheral blood leukocytes with anti-human CD33 (HIM3-4) biotin / streptavidin-APC.
Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) - BSA Free [NB500-508]

Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) - BSA Free [NB500-508]

Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) [NB500-508] - Analysis using the PE/Cy5 conjugate of NB500-508. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 K Isotype Control (open histogram) or Anti-Human CD33 PE-Cy5 (filled histogram). Cells in the monocyte gate were used for analysis.

Applications for Siglec-3/CD33 Antibody (HIM3-4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-5 ug/ml
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Siglec-3/CD33

CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Entrez Gene IDs

945 (Human)

Gene Symbol

CD33

Additional Siglec-3/CD33 Products

Product Documents for Siglec-3/CD33 Antibody (HIM3-4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Siglec-3/CD33 Antibody (HIM3-4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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