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Simple Plex AAV2 Cartridge

Catalog #: SPCKB-OT-005905 Datasheet / COA / SDS
Simple Plex AAV2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD19, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-OT-005905
ST01B-OT-005905
AAV2 Simple Plex Assay
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex AAV2 Cartridge Summary

Sample Type & Volume Required Per Well*
Bioprocess Samples (25 uL)
Sensitivity
0.9 10^6 capsids/mL
Assay Range
3.4 - 13,000 10^6 capsids/mL
Specificity
This assay recognizes intact AAV2 capsids. The AAV2 antibody (A20R) used in this assay cross-reacts with AAV3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of Adeno-Associated Virus type 2 (AAV2) intact capsids in bioprocess samples. This assay uses PROGEN's AAV2 (A20R) antibody.
  • Sensitivity: 0.9 x 106 capsids/mL
  • Range: 3.41 x 106 - 1.3 x 1010 capsids/mL

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bioprocess Samples

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 58 56
Mean (106 capsids/mL) 40 2103 42.2 2045
Standard Deviation 3.5 55.3 4.7 158
CV% 8.8 2.6 11.2 7.7

Recovery

Recovery was not necessary as AAV2 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of AAV2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
AAV2 Simple Plex Assay

Scientific Data

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AAV2 Simple Plex Assay

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

FAQs

  1. Do the AAV assays recognize fully-assembled capsids?

    • Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.

  2. Do the AAV assays recognize both natural and recombinant AAV?

    • Yes, the AAV assays detect both natural and recombinant AAV. 

  3. Does the AAV2 assay recognize other AAV serotypes?

    • This assay recognizes intact AAV2 capsids. The AAV2 antibody (A20R) used in this assay cross-reacts with AAV3.

  4. Do the Simple Plex AAV assays distinguish between full and empty capsids?

    • No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.

  5. What diluent is provided with the AAV assays?

    • The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use. 

  6. What is the minimum required dilution (MRD) for bio-process samples?

    • All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery. 

View all Simple Plex Assays FAQs

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