Simple Plex AAV2 Cartridge
Simple Plex AAV2 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
- Sensitivity: 0.9 x 106 capsids/mL
- Range: 3.41 x 106 - 1.3 x 1010 capsids/mL
|Intra-Assay Precision||Inter-Assay Precision|
|Mean (106 capsids/mL)||40||2103||42.2||2045|
Recovery was not necessary as AAV2 shows natural linearity data from a neat sample to a 1:16 dilution.
AAV2 Simple Plex Assay
Preparation and Storage
Do the AAV assays recognize fully-assembled capsids?
Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.
Do the AAV assays recognize both natural and recombinant AAV?
Yes, the AAV assays detect both natural and recombinant AAV.
Does the AAV2 assay recognize other AAV serotypes?
This assay recognizes intact AAV2 capsids. The AAV2 antibody (A20R) used in this assay cross-reacts with AAV3.
Do the Simple Plex AAV assays distinguish between full and empty capsids?
No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.
What diluent is provided with the AAV assays?
The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use.
What is the minimum required dilution (MRD) for bio-process samples?
All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.
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