Simple Plex Discovery Human Pancreatic Polypeptide Assay

R&D Systems | Catalog # SPCKB-PS-012305

Simple Plex Human PP assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

1.19 pg/mL

Assay Range

3.28-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Pancreatic Polypeptide Assay Summary

Simple Plex Discovery assay is for the detection of human Pancreatic Polypeptide in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Pancreatic Polypeptide/PP Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Pancreatic Polypeptide. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 42 42
Mean (pg/mL) 17.7 875 16.3 805
Standard Deviation 0.930 31.3 1.64 71.3
CV% 5.3 3.6 10.1 8.9

Recovery for Simple Plex Discovery Human Pancreatic Polypeptide Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 97 94-101
EDTA Plasma (n=2) 101 95-107
Heparin Plasma (n=2) 86 85-86
Serum (n=2) 91 87-95

Linearity

Samples containing and/or spiked with high concentrations of human Pancreatic Polypeptide were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human Pancreatic Polypeptide/PP Ella Assay

Scientific Data Images for Simple Plex Discovery Human Pancreatic Polypeptide Assay

Simple Plex Discovery Human Pancreatic Polypeptide/PP Ella Assay

Simple Plex Discovery Human Pancreatic Polypeptide/PP Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pancreatic Polypeptide/PP

Pancreatic polypeptide (PP) is an ~4 kDa, unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the PP hormone, an icosapeptide of unknown function, and a C-terminal prosequence. PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake.

Alternate Names

INN, Obinepitide, PNP, PP, PPY

Entrez Gene IDs

5539 (Human)

Gene Symbol

PPY

Additional Pancreatic Polypeptide/PP Products

Product Documents for Simple Plex Discovery Human Pancreatic Polypeptide Assay

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human Pancreatic Polypeptide Assay

For research use only

Related Research Areas

Nutrition and Appetite
Obesity

Citations for Simple Plex Discovery Human Pancreatic Polypeptide Assay

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