Simple Plex Discovery Human TGF-alpha Assay

R&D Systems | Catalog # SPCKB-PS-012306

Simple Plex Human TGF-a assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.17 pg/mL

Assay Range

0.46-1730 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human TGF-alpha Assay Summary

Simple Plex Discovery assay is for the detection of human Transforming Growth Factor alpha (TGF-alpha ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TGF-alpha Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human TGF-alpha.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 42 42
Mean (pg/mL) 6.03 283 5.47 261
Standard Deviation 0.174 6.18 0.522 17.9
CV% 2.9 2.2 9.5 6.9

Recovery for Simple Plex Discovery Human TGF-alpha Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 97 97-98
EDTA Plasma (n=3) 96 91-101
Heparin Plasma (n=2) 99 98-100
Serum (n=2) 98 94-102

Linearity

Samples containing and/or spiked with high concentrations of human TGF-alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human TGF-alpha Ella Assay

Scientific Data Images for Simple Plex Discovery Human TGF-alpha Assay

Simple Plex Discovery Human TGF-alpha Ella Assay

Simple Plex Discovery Human TGF-alpha Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-alpha

TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors with soluble forms released by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and plays a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. TGF-alpha binds and activates the EGF receptor. It induces fibroblast proliferation, epithelial development, keratinocyte migration, and angiogenesis.

Long Name

Transforming Growth Factor alpha

Alternate Names

TGFA, TGFalpha

Entrez Gene IDs

7039 (Human)

Gene Symbol

TGFA

Additional TGF-alpha Products

Product Documents for Simple Plex Discovery Human TGF-alpha Assay

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human TGF-alpha Assay

For research use only

Citations for Simple Plex Discovery Human TGF-alpha Assay

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