Simple Plex Discovery Human CCL13/MCP-4 Assay New

Catalog #: SPCKB-PS-014652 Datasheet / COA / SDS

Simple Plex Human MCP-4 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-014652
SPCKA-PS-014652
SPCKC-PS-014652

Control Products Available

897220: Simple Plex Discovery Control for Human CCL13/MCP-4

Simple Plex Discovery Human MCP-4 Assay Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human CCL13/MCP-4 Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.05 pg/mL

Assay Range

0.4 - 1,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human MCP-4.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human CCL13/MCP-4 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one MCP-4 assay

Inter-Assay Precision (Precision between assays) Replicates of each MCP-4 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human MCP-4

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	37	36
Mean (pg/mL)	14.1	752	13.2	684
Standard Deviation	0.517	19.4	0.904	62.9
CV%	3.7	2.6	6.8	9.2

Recovery

Recovery for the human MCP-4 assays was evaluated at three different spiked concentrations of MCP-4 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	98	96-101

Linearity

Samples containing and/or spiked with high concentrations of MCP-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human MCP-4 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human MCP-4 Assay. Assay range is 0.42-1600 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL13/MCP-4

Human MCP-4, also known as CCL13, encodes a 98 amino acid (aa) precursor protein with a 23 aa hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature MCP-4. Mature MCP-4 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins.

Entrez Gene IDs:

6357 (Human)

Alternate Names:

C-C motif chemokine 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13