Simple Plex Discovery Human CCL13/MCP-4 Assay
R&D Systems | Catalog # SPCKB-PS-014652
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human CCL13/MCP-4 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one MCP-4 assay.
Inter-Assay Precision (Precision between assays) Replicates of each MCP-4 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human MCP-4.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 37 | 36 |
| Mean (pg/mL) | 14.1 | 752 | 13.2 | 684 |
| Standard Deviation | 0.517 | 19.4 | 0.904 | 62.9 |
| CV% | 3.7 | 2.6 | 6.8 | 9.2 |
Recovery for Simple Plex Discovery Human CCL13/MCP-4 Assay
Recovery for the human MCP-4 assays was evaluated at three different spiked concentrations of MCP-4 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 98 | 96-101 |
Linearity
Samples containing and/or spiked with high concentrations of MCP-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human CCL13/MCP-4 Assay
Simple Plex Discovery Human MCP-4 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human MCP-4 Assay. Assay range is 0.42-1600 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CCL13/MCP-4
Human MCP-4, also known as CCL13, encodes a 98 amino acid (aa) precursor protein with a 23 aa hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature MCP-4. Mature MCP-4 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins.
Additional CCL13/MCP-4 Products
Product Documents for Simple Plex Discovery Human CCL13/MCP-4 Assay
Certificate of Analysis
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Product Specific Notices for Simple Plex Discovery Human CCL13/MCP-4 Assay
For research use only
Related Research Areas
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Associated Pathways
