Simple Plex Discovery Human CD155/PVR Assay New

Catalog #: SPCKB-PS-012015 Datasheet / COA / SDS

Simple Plex Human CD155/PVR assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-012015
SPCKA-PS-012015
SPCKC-PS-012015

Control Products Available

897036: Simple Plex Discovery Control for Human CD155/PVR

Simple Plex Discovery Human CD155/PVR Ella Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human CD155/PVR Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

7.29 pg/mL

Assay Range

13.1 - 50,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human CD155/PVR.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human CD155/PVR in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	28	28
Mean (pg/mL)	326	15314	320	14298
Standard Deviation	12.9	494	32.9	1349
CV%	4	3.2	10.3	9.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	91	89-93

Linearity

Samples containing and/or spiked with high concentrations of recombinant human CD155/PVR were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human CD155/PVR Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor) and Necl-5 (Nectin-like 5), is a 70 kDa type I transmembrane glycoprotein that is a member of the nectin-like family. CD155 is expressed in all normal tissues and highly expressed in tumor cells of epithelial and neuronal origin. CD155 was orginally isolated based on its ability to mediate poliovirus attachment to host cells.

Long Name:

Poliovirus Receptor

Entrez Gene IDs:

5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)

Alternate Names:

CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4