Simple Plex Discovery Human CD155/PVR Assay

R&D Systems | Catalog # SPCKB-PS-012015

Simple Plex Human CD155/PVR assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

7.29 pg/mL

Assay Range

13.11-50000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human CD155/PVR Assay Summary

Simple Plex Discovery assay is for the detection of human CD155/PVR in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin). 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human CD155/PVR. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 28 28
Mean (pg/mL) 326 15,314 320 14,298
Standard Deviation 12.9 494 32.9 1349
CV% 4.0 3.2 10.3 9.4

Recovery for Simple Plex Discovery Human CD155/PVR Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 91 89-93

Linearity

Samples containing and/or spiked with high concentrations of recombinant human CD155/PVR were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human CD155/PVR Ella Assay

Scientific Data Images for Simple Plex Discovery Human CD155/PVR Assay

Simple Plex Discovery Human CD155/PVR Ella Assay

Simple Plex Discovery Human CD155/PVR Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor) and Necl-5 (Nectin-like 5), is a 70 kDa type I transmembrane glycoprotein that is a member of the nectin-like family. CD155 is expressed in all normal tissues and highly expressed in tumor cells of epithelial and neuronal origin. CD155 was orginally isolated based on its ability to mediate poliovirus attachment to host cells.

Long Name

Poliovirus Receptor

Alternate Names

CD155, HVED, Necl-5, PVR, PVS

Entrez Gene IDs

5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)

Gene Symbol

PVR

Additional CD155/PVR Products

Product Documents for Simple Plex Discovery Human CD155/PVR Assay

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Product Specific Notices for Simple Plex Discovery Human CD155/PVR Assay

For research use only

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