Simple Plex Discovery Human CG beta Assay

R&D Systems | Catalog # SPCKB-PS-012702

Simple Plex Human CG beta assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.3 pg/mL

Assay Range

1.31-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human CG beta Assay Summary

Simple Plex Discovery assay is for the detection of human Chorionic Gonadotropin Beta Chain (CG beta ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Chorionic Gonadotropin beta Chain (HCG beta) Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human CG beta. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 24 25
Mean (pg/mL) 17.3 664 15.0 694
Standard Deviation 0.763 19.3 1.69 35.8
CV% 4.4 2.9 11.3 5.2

Recovery for Simple Plex Discovery Human CG beta Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 102 100-103
EDTA Plasma (n=2) 109 100-117
Heparin Plasma (n=2) 108 103-113
Serum (n=2) 116 115-116

Linearity

Samples containing and/or spiked with high concentrations of human CG beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human CG beta Ella Assay

Scientific Data Images for Simple Plex Discovery Human CG beta Assay

Simple Plex Discovery Human CG beta Ella Assay

Simple Plex Discovery Human CG beta Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chorionic Gonadotropin beta Chain (HCG beta)

HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.

Long Name

Chorionic Gonadotropin beta Chain

Alternate Names

CG-Beta, CGB, CGB3, CGB5, CGB8, Choriogonadotropin Subunit Beta

Entrez Gene IDs

1082 (Human)

Gene Symbol

CGB3

Additional Chorionic Gonadotropin beta Chain (HCG beta) Products

Product Documents for Simple Plex Discovery Human CG beta Assay

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human CG beta Assay

For research use only

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