Simple Plex Discovery Human Dopa Decarboxylase/DDC

R&D Systems | Catalog # SPCKB-PS-015351

Simple Plex Human DDC assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

0.41 pg/mL

Assay Range

0.63-6000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Discovery Human Dopa Decarboxylase/DDC Summary

Simple Plex Discovery assay is for the detection of human Dopa Decarboxylase (DDC) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Dopa Decarboxylase/DDC Simple Plex »

Compatible Instruments

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human DDC

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one DDC assay.

Inter-Assay Precision (Precision between assays) Replicates of each DDC control were tested in multiple assays performed by at least three technicians using two lots of reagents for human DDC.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 35 35
Mean (pg/mL) 19.8 916 18.5 858
Standard Deviation 0.494 28.3 1.73 56.9
CV% 2.5 3.1 9.3 6.6

Recovery for Simple Plex Discovery Human Dopa Decarboxylase/DDC

Recovery for the human DDC assays was evaluated at three different spiked concentrations of DDC protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 103 102-103
Cerebrospinal Fluid (n=2) 105 104-105

Linearity

Samples containing and/or spiked with high concentrations of DDC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human DDC Assay Linearity

Scientific Data Images for Simple Plex Discovery Human Dopa Decarboxylase/DDC

Simple Plex Discovery Human DDC Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human DDC Assay. Assay range is 0.63-6000 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Dopa Decarboxylase/DDC

Dopa decarboxylase (DDC) is a group II decarboxylase that catalyzes the decarboxylation of aromatic L-amino acids. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. Deficiency of DDC is associated with severe developmental delay, oculogyric crises and autonomic dysfunction.

Alternate Names

AADC, DDC

Entrez Gene IDs

1644 (Human)

Gene Symbol

DDC

Additional Dopa Decarboxylase/DDC Products

Product Documents for Simple Plex Discovery Human Dopa Decarboxylase/DDC

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human Dopa Decarboxylase/DDC

For research use only

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