Simple Plex Discovery Human Dopa Decarboxylase/DDC New

Catalog #: SPCKB-PS-015351 Datasheet / COA / SDS

Simple Plex Human DDC assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-015351
SPCKA-PS-015351
SPCKC-PS-015351

Control Products Available

897226: Simple Plex Discovery Control for Human DDC

Simple Plex Discovery Human DDC Assay Standard Curve

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All Dopa Decarboxylase/DDC Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human Dopa Decarboxylase/DDC Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL), Cerebrospinal Fluid (5 uL)

Sensitivity

0.41 pg/mL

Assay Range

0.6 - 6,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant human DDC

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Dopa Decarboxylase (DDC) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one DDC assay

Inter-Assay Precision (Precision between assays) Replicates of each DDC control were tested in multiple assays performed by at least three technicians using two lots of reagents for human DDC

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision	Inter-Assay Precision
Sample	1	1	2	2
n	16	35	16	35
Mean (pg/mL)	19.8	18.5	916	858
Standard Deviation	0.494	1.73	28.3	56.9
CV%	2.5	9.3	3.1	6.6

Recovery

Recovery for the human DDC assays was evaluated at three different spiked concentrations of DDC protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	103	102-103
Cerebrospinal Fluid (n=2)	105	104-105

Linearity

Samples containing and/or spiked with high concentrations of DDC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human DDC Assay. Assay range is 0.63-6000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Dopa Decarboxylase/DDC

Dopa decarboxylase (DDC) is a group II decarboxylase that catalyzes the decarboxylation of aromatic L-amino acids. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. Deficiency of DDC is associated with severe developmental delay, oculogyric crises and autonomic dysfunction.

Entrez Gene IDs:

1644 (Human)

Alternate Names:

AADC; AADCaromatic-L-amino-acid decarboxylase; DDC; dopa decarboxylase (aromatic L-amino acid decarboxylase); Dopa Decarboxylase; EC 4.1.1; EC 4.1.1.28