Simple Plex Discovery Human GDNF Assay
R&D Systems | Catalog # SPCKB-PS-012636
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human GDNF Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 24 | 24 |
| Mean (pg/mL) | 26.6 | 1123 | 21.3 | 1078 |
| Standard Deviation | 1.76 | 66.6 | 1.64 | 110 |
| CV% | 6.6 | 5.9 | 7.7 | 10.2 |
Recovery for Simple Plex Discovery Human GDNF Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 106 | 105-106 |
| EDTA Plasma (n=2) | 83 | 72-93 |
| Heparin Plasma (n=2) | 87 | 83-90 |
| Serum (n=10) | 81 | 68-94 |
Linearity
Samples containing and/or spiked with high concentrations of human GDNF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human GDNF Assay
Simple Plex Discovery Human GDNF Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: GDNF
GDNF (Glial Cell Line-derived Neurotrophic Factor) is a secreted protein that promotes the survival of motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. It is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. GDNF binding to GFR alpha 1 induces the recruitment of Ret, NCAM-1/CD56, various integrins, Syndecan-3, or N-Cadherin. GDNF-based therapies show promise in several neurodegenerative disorders, particularly Parkinson’s disease.
Long Name
Alternate Names
Gene Symbol
Additional GDNF Products
Product Documents for Simple Plex Discovery Human GDNF Assay
Product Specific Notices for Simple Plex Discovery Human GDNF Assay
For research use only
Related Research Areas
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Associated Pathways
