Simple Plex Discovery Human LDLR Assay
R&D Systems | Catalog # SPCKB-PS-012639
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human LDLR Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 23 |
| Mean (pg/mL) | 377 | 19822 | 407 | 20382 |
| Standard Deviation | 15.5 | 918 | 45.7 | 2018 |
| CV% | 4.1 | 4.6 | 11.2 | 9.9 |
Recovery for Simple Plex Discovery Human LDLR Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 106 | 106-106 |
Linearity
Samples containing and/or spiked with high concentrations of human LDLR were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human LDLR Assay
Simple Plex Discovery Human LDLR Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: LDLR
LDLR (Low Density Lipoprotein Receptor) is a widely expressed cell surface scavenger receptor. LDLR binds ApoB and ApoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively. Hepatocyte LDLR is responsible for endocytosis and clearing of most plasma LDL cholesterol. At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDLR to the cell surface. Lack of LDLR expression or function causes familial hypercholesterolemia (FH). The protease PCSK9 can also cause increased plasma cholesterol by promoting LDLR degradation rather than recycling to the cell surface.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional LDLR Products
Product Documents for Simple Plex Discovery Human LDLR Assay
Product Specific Notices for Simple Plex Discovery Human LDLR Assay
For research use only
Related Research Areas
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Associated Pathways
