Simple Plex Discovery Human PD-1 Assay
R&D Systems | Catalog # SPCKB-PS-012129
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human PD-1 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 29 | 28 |
| Mean (pg/mL) | 49.6 | 2260 | 45.0 | 2200 |
| Standard Deviation | 5.11 | 175 | 4.65 | 193 |
| CV% | 10.3 | 7.8 | 10.3 | 8.8 |
Recovery for Simple Plex Discovery Human PD-1 Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 105 | 98-112 |
Linearity
Scientific Data Images for Simple Plex Discovery Human PD-1 Assay
Simple Plex Discovery Human PD-1 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: PD-1
PD-1 (Programmed Death-1 receptor) is a key regulator of the threshold of immune response and peripheral immune tolerance. It is expressed on activated T cells, B cells, monocytes, and dendritic cells and binds to PD-L1 or PD-L2. PD-1 ligation induces co-inhibitory signals in T cells promoting their apoptosis, anergy, and functional exhaustion. Many tumors overexpress PD-L1 to induce this inhibitory signaling pathway in immune cells. When PD-1 expressing immune cells come into contact with a tumor cell expressing PD-L1, this interaction blocks activation and prevents an immune response. A new class of anti-cancer therapies disrupt these co-inhibitory signals taking the breaks off the host immune system thereby enhancing anti-tumor immunity. Recent success of several antibody therapeutics has spurred great interest in PD-1 and PD-L1 in addition to other co-inhibitory immune checkpoint proteins.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional PD-1 Products
Product Documents for Simple Plex Discovery Human PD-1 Assay
Certificate of Analysis
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Product Specific Notices for Simple Plex Discovery Human PD-1 Assay
For research use only
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