Simple Plex Discovery Human PLA2G7/PAF-AH/L-PLA2 Cartridge

R&D Systems | Catalog # SPCKB-PS-016136

Simple Plex Discovery Human PLA2G7/PAF-AH/L-PLA2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

6.83 pg/mL

Assay Range

27.8-42424 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human PLA2G7/PAF-AH/L-PLA2 Cartridge Summary

Simple Plex Discovery assay is for the detection of human Phospholipase A2 Group VII (PLA2G7) protein, also known as platelet-activating factor acetylhydrolase (PAF-AH) and lipoprotein-associated phospholipase A2 (Lp-PLA2), in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) samples using Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all PLA2G7/PAF-AH/Lp-PLA2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human PLA2G7.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one PLA2G7/PAF-AH/Lp-PLA2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each PLA2G7/PAF-AH/Lp-PLA2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human PLA2G7/PAF-AH/Lp-PLA2.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 22 22
Mean (pg/mL) 298 14350 305 15346
Standard Deviation 9.05 529 34.2 1338
CV% 3.0 3.7 11.2 8.7

Recovery for Simple Plex Discovery Human PLA2G7/PAF-AH/L-PLA2 Cartridge

Recovery for the human PLA2G7/PAF-AH/Lp-PLA2 assays was evaluated at three different spiked concentrations of PLA2G7/PAF-AH/Lp-PLA2 protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 106 105-106

Linearity

Samples containing and/or spiked with high concentrations of PLA2G7/PAF-AH/Lp-PLA2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human PLA2G7/PAF-AH/Lp-PLA2 Assay Linearity

Scientific Data Images for Simple Plex Discovery Human PLA2G7/PAF-AH/L-PLA2 Cartridge

Simple Plex Discovery Human PLA2G7/PAF-AH/Lp-PLA2 Assay Standard Curve

Simple Plex Discovery Human PLA2G7/PAF-AH/Lp-PLA2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human PLA2G7/PAF-AH/Lp-PLA2 Assay. Assay range is 27.8-42,425 pg/mL

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Background: PLA2G7/PAF-AH/Lp-PLA2

Secretory phopholipase A2 (PLA2) enzymes cleave an acyl ester bond in the sn-2 position of glycerophospholipids. These extracellular proteins have a high disulfide bond content, low molecular mass (14 kDa), and require mM levels of Ca2+ for catalysis. They play a crucial role in the generation of arachidonates and eicosanoids, and have a number of biological actions including immunological responses, inflammation, cellular proliferation, vasoconstriction, and bronchioconstriction.

Long Name

Phospholipase A2 Group VII

Alternate Names

LDL-PLA2, Lp-PLA2, LpPLA2, PAF-AH

Entrez Gene IDs

7941 (Human); 27226 (Mouse); 301265 (Rat)

Gene Symbol

PLA2G7

Additional PLA2G7/PAF-AH/Lp-PLA2 Products

Product Documents for Simple Plex Discovery Human PLA2G7/PAF-AH/L-PLA2 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human PLA2G7/PAF-AH/L-PLA2 Cartridge

For research use only

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