Simple Plex Discovery Human TSLP Assay

R&D Systems | Catalog # SPCKB-PS-011826

Simple Plex Human TSLP assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.09 pg/mL

Assay Range

0.21-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human TSLP Assay Summary

Simple Plex Discovery assay is for the detection of human Thymic Stromal Lymphopoietin (TSLP) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TSLP Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human TSLP. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 25 27
Mean (pg/mL) 11.6 546 12.2 583
Standard Deviation 0.367 17.9 0.885 30.3
CV% 3.2 3.3 7.3 5.2

Recovery for Simple Plex Discovery Human TSLP Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 110 107-113
EDTA Plasma (n=2) 86 84-87
Heparin Plasma (n=2) 99 96-102
Serum (n=2) 92 92-92

Linearity

Samples containing and/or spiked with high concentrations of recombinant human TSLP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery TSLP Ella Assay

Scientific Data Images for Simple Plex Discovery Human TSLP Assay

Simple Plex Discovery TSLP Ella Assay

Simple Plex Discovery TSLP Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TSLP

TSLP (Thymic Stromal Lymphopoietin) is a secreted cytokine that signals through a complex of TSLP R and IL-7 R alpha/CD127. It acts on thymic dendritic cells which convert potentially autoreactive T cells into regulatory T cells. TSLP promotes fetal liver pro-B cell proliferation as well as large pre-B cell differentiation and B cell lymphopoiesis in adult bone marrow. It is produced by allergen challenged keratinocytes and mucosal epithelial cells. It enhances Th2 biased allergic inflammation by limiting Th1/Th17 inflammation, promoting Th2 differentiation, and enhancing the function of basophils, eosinophils, and mast cells.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)

Gene Symbol

TSLP

Additional TSLP Products

Product Documents for Simple Plex Discovery Human TSLP Assay

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human TSLP Assay

For research use only

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