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Simple Plex HIV-1 p24 2nd Gen Cartridge

R&D Systems | Catalog # SPCKB-CS-009216

Simple Plex HIV-1 p24 2nd Gen assay kit for use on Ella instrument. Contains cartridge, sample diluent SD30, and wash buffer. Sample type indicated with CS (Cell Culture Supernates).
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL)

Sensitivity

5.1 pg/mL

Assay Range

11.7-112000 pg/mL (Cell Culture Supernates)

Simple Plex HIV-1 p24 2nd Gen Cartridge Summary

Simple Plex Assay is for the detection of HIV-1 p24 in cell culture supernatant (CCS) in Sample Diluent SD30. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all HIV-1 Gag p24 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Specificity

This assay recognizes HIV-1 p24.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 29 29
Mean (µL) 782 36099 856 37492
Standard Deviation 38.0 2120 93.1 2511
CV% 4.9 5.9 10.9 6.7

Recovery for Simple Plex HIV-1 p24 2nd Gen Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media Pretreated (n=7) 101 91-111
Cell Culture Media Untreated (n=7) 104 90-115

Linearity

Samples containing and/or spiked with high concentrations of HIV-1 p24 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex HIV-1 p24 Assay Linearity

Scientific Data Images for Simple Plex HIV-1 p24 2nd Gen Cartridge

Simple Plex HIV-1 p24 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex HIV-1 p24 Assay. Assay range is 11.7-112,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Entrez Gene IDs

155030 (Viral)

Gene Symbol

GAG

Additional HIV-1 Gag p24 Products

Product Documents for Simple Plex HIV-1 p24 2nd Gen Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex HIV-1 p24 2nd Gen Cartridge

For research use only

Related Research Areas

Infectious Diseases

Citations for Simple Plex HIV-1 p24 2nd Gen Cartridge

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