Simple Plex Human alpha-Fetoprotein/AFP Cartridge

R&D Systems | Catalog # SPCKB-PS-001710

Simple Plex Human alpha-Fetoprotein/AFP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 nL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

1.21 pg/mL

Assay Range

5.24-50000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human alpha-Fetoprotein/AFP Cartridge Summary

Simple Plex assay is for the detection of human alpha -Fetoprotein/AFP in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all alpha-Fetoprotein/AFP Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human AFP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one AFP assay.

Inter-Assay Precision (Precision between assays) Replicates of each AFP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human AFP.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 35 36
Mean (pg/mL) 269 12,419 306 13,577
Standard Deviation 9.94 464 31.2 1368
CV% 3.7 3.7 10.2 10.1

Recovery for Simple Plex Human alpha-Fetoprotein/AFP Cartridge

Recovery was not necessary as human AFP shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human AFP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human AFP Assay Linearity

Scientific Data Images for Simple Plex Human alpha-Fetoprotein/AFP Cartridge

Simple Plex Human AFP Assay Standard Curve

Simple Plex Human AFP Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human AFP Assay. Assay range is 5.24-50,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha-Fetoprotein/AFP

alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human); 11576 (Mouse); 24177 (Rat)

Gene Symbol

AFP

Additional alpha-Fetoprotein/AFP Products

Product Documents for Simple Plex Human alpha-Fetoprotein/AFP Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human alpha-Fetoprotein/AFP Cartridge

For research use only

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