Simple Plex Human Amphiregulin Cartridge
R&D Systems | Catalog # SPCKB-PS-001181
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Amphiregulin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Amphiregulin assay.
Inter-Assay Precision (Precision between assays) Replicates of each Amphiregulin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Amphiregulin.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 69 | 69 |
| Mean (pg/mL) | 73.7 | 3225 | 68.1 | 3228 |
| Standard Deviation | 3.19 | 236 | 7.01 | 285 |
| CV% | 4.3 | 7.3 | 10.3 | 8.8 |
Recovery for Simple Plex Human Amphiregulin Cartridge
Recovery for the human Amphiregulin assays was evaluated at three different spiked concentrations of Amphiregulin protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 83 | 81-85 |
| Heparin Plasma (n=4) | 83 | 79-86 |
| Serum (n=4) | 84 | 83-85 |
Linearity
Samples containing and/or spiked with high concentrations of human Amphiregulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Amphiregulin Cartridge
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Amphiregulin Assay. Assay range is 5.41-51,610 pg/mL.
Preparation and Storage
Shipping
Stability & Storage
Background: Amphiregulin
Amphiregulin (AR) is an EGF family growth factor that is released as a soluble protein following proteolytic cleavage of its transmembrane precursor. Amphiregulin is expressed by numerous carcinoma cell lines and epithelial cells of the colon, stomach, breast, ovary and kidney. It acts through ErbB family of receptor to stimulate the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. Amphiregulin can also inhibit the growth of certain tumor cells.
Additional Amphiregulin Products
Product Documents for Simple Plex Human Amphiregulin Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Human Amphiregulin Cartridge
For research use only
Related Research Areas
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Associated Pathways
