Simple Plex Human Amyloid Beta (aa1-40) Cartridge

R&D Systems | Catalog # SPCKB-PS-008817

Simple Plex Human Amyloid Beta (aa1-40) assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

7.8 pg/mL

Assay Range

24.5-2400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human Amyloid Beta (aa1-40) Cartridge Summary

Simple Plex assay for the detection of human Amyloid beta (amino acids 1-40) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Amyloid beta Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Amyloid beta (aa1-40).

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay)

Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 35 36
Mean (pg/mL) 35.2 1582 34.6 1524
Standard Deviation 1.27 73.4 3.58 137
CV% 3.6 4.6 10.4 9.0

Recovery for Simple Plex Human Amyloid Beta (aa1-40) Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 98 94-103
EDTA Plasma (n=4) 91 86-94
Heparin Plasma (n=4) 88 83-95
Serum (n=4) 93 87-104

Linearity

Samples containing and/or spiked with high concentrations of human Amyloid beta (aa1-40) were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human Amyloid Beta (aa1-40) Cartridge

Human Amyloid beta (aa1-40) Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Amyloid beta

Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.

Alternate Names

Abeta, beta-Amyloid Peptide

Entrez Gene IDs

351 (Human); 11820 (Mouse)

Gene Symbol

APP

Additional Amyloid beta Products

Product Documents for Simple Plex Human Amyloid Beta (aa1-40) Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Amyloid Beta (aa1-40) Cartridge

For research use only

Citations for Simple Plex Human Amyloid Beta (aa1-40) Cartridge

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