Simple Plex Human Angiopoietin-like 3 Cartridge

Catalog #: SPCKB-PS-008169 Datasheet / COA / SDS
Simple Plex Human Angiopoietin-like Protein 3/ANGPTL3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKA-PS-008169
SPCKB-PS-008169
SPCKC-PS-008169
Control Products Available
Human Angiopoietin-like 3 Ella Assay
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Simple Plex Human Angiopoietin-like 3 Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
15.7 pg/mL
Assay Range
15.7 - 24,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human ANGPTL3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Angiopoietin-like 3 (ANGPTL3) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 105 101-109

Linearity

Samples containing and/or spiked with high concentrations of human ANGPTL3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Human Angiopoietin-like 3 Ella Assay Linearity

Scientific Data

Human Angiopoietin-like 3 Ella Assay Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Entrez Gene IDs:
27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names:
AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2

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