Simple Plex Human B7-H1/PD-L1 2nd Gen Cartridge
R&D Systems | Catalog # SPCKB-PS-003468
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human B7-H1/PD-L1 2nd Gen Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one B7-H1 assay.
Inter-Assay Precision (Precision between assays) Replicates of each B7-H1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human B7-H1.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 49 | 48 |
| Mean (pg/mL) | 74.9 | 3685 | 71.7 | 3750 |
| Standard Deviation | 6.64 | 225 | 6.99 | 253 |
| CV% | 8.9 | 6.1 | 9.7 | 6.8 |
Recovery for Simple Plex Human B7-H1/PD-L1 2nd Gen Cartridge
Recovery was not necessary as human B7-H1 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human B7-H1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human B7-H1/PD-L1 2nd Gen Cartridge
Simple Plex Human B7-H1 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human B7-H1 Assay. Assay range is 0.56-5332 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: PD-L1/B7-H1
PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 protein binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells. Many tumors overexpress PD-L1 protein making the PD-1:PD-L1 interaction central to several successful cancer immunotherapy approaches.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional PD-L1/B7-H1 Products
Product Documents for Simple Plex Human B7-H1/PD-L1 2nd Gen Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human B7-H1/PD-L1 2nd Gen Cartridge
For research use only
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