Simple Plex Human Beta-NGF Cartridge
R&D Systems | Catalog # SPCKB-PS-000623
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Beta-NGF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 16 | 17 |
| Mean (pg/mL) | 19.3 | 1131 | 19.6 | 1034 |
| Standard Deviation | 1.68 | 101 | 2.22 | 96.5 |
| CV% | 8.7 | 8.9 | 11.3 | 9.3 |
Recovery for Simple Plex Human Beta-NGF Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 89 | 74-110 |
| Heparin Plasma (n=4) | 96 | 88-109 |
| Serum (n=4) | 90 | 83-95 |
Linearity
Samples containing and/or spiked with high concentrations of human beta -NGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Beta-NGF Cartridge
Human beta -NGF Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: beta-NGF
Beta-NGF (nerve growth factor) is a secreted dimeric neurotrophin that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. It can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. Beta-NGF additionally promotes mast cell and basophil proliferation and activation and induces the differentiation of B cells
Long Name
Alternate Names
Gene Symbol
Additional beta-NGF Products
Product Documents for Simple Plex Human Beta-NGF Cartridge
Product Specific Notices for Simple Plex Human Beta-NGF Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Human Beta-NGF Cartridge
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