Simple Plex Human Caspase-1 Cartridge (2nd Gen)
Simple Plex Human Caspase-1 Cartridge (2nd Gen) Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 29 | 30 |
| Mean (pg/mL) | 2.3 | 117 | 2.28 | 125 |
| Standard Deviation | 0.104 | 4.09 | 0.259 | 12.2 |
| CV% | 4.5 | 3.5 | 11.4 | 9.8 |
Recovery
Recovery for the human Caspace-1 assays was evaluated at three different spiked concentrations of Caspace-1 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cerebrospinal Fluid (n=5) | 116 | 103-123 |
| EDTA Plasma (n=4) | 90 | 78-101 |
| Heparin Plasma (n=4) | 90 | 82-97 |
| Serum (n=4) | 91 | 83-97 |
Linearity
Scientific Data
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Human Caspase-1 Simple Plex Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Caspace-1 Assay. Assay range is 0.11-422 pg/mL
Product Datasheets
Preparation and Storage
Background: Caspase-1
Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).
Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.
FAQs
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