Simple Plex Human Caspase-1 Cartridge (2nd Gen)

R&D Systems | Catalog # SPCKB-PS-003613

Simple Plex Human Caspase-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

80 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

0.04 pg/mL

Assay Range

0.11-422 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human Caspase-1 Cartridge (2nd Gen) Summary

Simple Plex assay is for the detection of human Caspase-1/ICE in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin, and cerebrospinal fluid (CSF) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Caspase-1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Caspase-1. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Caspase-1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Caspace-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Caspace-1.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 29 30
Mean (pg/mL) 2.30 117 2.28 125
Standard Deviation 0.104 4.09 0.259 12.2
CV% 4.5 3.5 11.4 9.8

Recovery for Simple Plex Human Caspase-1 Cartridge (2nd Gen)

Recovery for the human Caspace-1 assays was evaluated at three different spiked concentrations of Caspace-1 protein.

Sample Type Average % Recovery Range %
Cerebrospinal Fluid (n=5) 116 103-123
EDTA Plasma (n=4) 90 78-101
Heparin Plasma (n=4) 90 82-97
Serum (n=4) 91 83-97

Linearity

Samples containing and/or spiked with high concentrations of Caspace-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human Caspace-1 Assay Linearity

Scientific Data Images for Simple Plex Human Caspase-1 Cartridge (2nd Gen)

Simple Plex Human Caspace-1 Assay Standard Curve

Human Caspase-1 Simple Plex Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Caspace-1 Assay. Assay range is 0.11-422 pg/mL

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Caspase-1

Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa and two 10 kDa subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine.

 

Alternate Names

CASP1, Caspase1, ICE

Entrez Gene IDs

834 (Human)

Gene Symbol

CASP1

Additional Caspase-1 Products

Product Documents for Simple Plex Human Caspase-1 Cartridge (2nd Gen)

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Caspase-1 Cartridge (2nd Gen)

For research use only

Citations for Simple Plex Human Caspase-1 Cartridge (2nd Gen)

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human Caspase-1 Cartridge (2nd Gen)

There are currently no reviews for this product. Be the first to review Simple Plex Human Caspase-1 Cartridge (2nd Gen) and earn rewards!

Have you used Simple Plex Human Caspase-1 Cartridge (2nd Gen)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card