Simple Plex Human CCL16/HCC-4 Cartridge

R&D Systems | Catalog # SPCKB-PS-000336

Simple Plex Human CCL16/HCC-4 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 µL), EDTA Plasma (5 µL), Heparin Plasma (5 µL)

Sensitivity

2.2 pg/mL

Assay Range

7.86-12000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CCL16/HCC-4 Cartridge Summary

Simple Plex assay is for the detection of human Hemofiltrate CC Chemokine 4 (HCC-4) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human HCC-4.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one HCC-4 assay.

Inter-Assay Precision (Precision between assays) Replicates of each HCC-4 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human HCC-4.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 19 18
Mean (pg/mL) 81.9 3709 91.3 4117
Standard Deviation 5.27 252 9.86 538
CV% 6.4 6.8 10.8 13.1

Recovery for Simple Plex Human CCL16/HCC-4 Cartridge

Recovery was not necessary as human HCC-4 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human HCC-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human HCC-4 Assay Linearity

Scientific Data Images for Simple Plex Human CCL16/HCC-4 Cartridge

Simple Plex Human HCC-4 Assay Standard Curve

Simple Plex Human HCC-4 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human HCC-4 Assay. Assay range is 7.86-12,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL16/HCC-4

Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1.

Alternate Names

HCC-4, HCC4, LEC, LMC, NCC-4

Entrez Gene IDs

6360 (Human)

Gene Symbol

CCL16

Additional CCL16/HCC-4 Products

Product Documents for Simple Plex Human CCL16/HCC-4 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human CCL16/HCC-4 Cartridge

For research use only

Citations for Simple Plex Human CCL16/HCC-4 Cartridge

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